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1987, 01, 29-38
干旱对小麦幼苗膜脂过氧化及保护酶的影响
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本实验用不同抗旱性的春小麦品种(干旱敏感的甘麦8号和抗旱的红农1号)作为分析材料。种子萌发到幼苗培养的过程是在控制的条件下完成的。第七天时,用高渗培养液(PEG6000)进行处理。本文研究了水分胁迫对小麦叶片相对含水量(RWC)、质膜透性、膜脂过氧化水平和超氧化物岐化酶(SOD)及过氧化氢酶活性的影响。

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Abstract:

The different drought-resistant spring wheat cultivars(drought snsitive Gabsu No.8 and drought-resistant Hongnu No.1)were used in the experi- ments.The process from seed germination to seedling culture was carried out under controlled conditions.On the seventh day,the seedlings were treated with hypertonic culture solution(PEG6000). In the present paper,the effect of water stress on the relative water content (RWC),on the permeability of plasma membrane,on the level of lipid per- oxidation and on the activities of superoxide dismutase(SOD)and catalase from the wheat leaves,was studied. RWC decreased with the increase of water stress.RWC of Hongnu No.1 decreased more slowly than that of Gansu No.8 under the same water stress. The activities of SOD and catalase of the leaves of the both cuitivars increas- ed under mild water stress.Correspondingly,the permeability of plasma mem- brane and the level of lipid peroxidation increased little.Under heavy water stress,the activity of SOD decreased,while the activity of catalase increased continously.Consequently,the permeability of plasma membrane and the level of lipid peroxidation increased greatly.The activities of SOD and catalase of drought-resistant cvltivar increased more than that of drought-sensitive culti- var,but the permeability of plasma membrane and the level of lipid peroxida- tion of the former were lower than those of the latter.Therefore,there appeared a good correlation between the abiity of a tisve to control lipid peroxidation and its ability to retain solutes.It suggested that at least water-stress-induced membrane damage is partly the result of biological free radical-initiated lipid peroxidation,and the ability of plant tissue to mobilize enzymatic defense against uncontrolled lipid peroxidation may be an important facet of the drought resistance.

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参考文献

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[1]王宝山,赵思齐.干旱对小麦幼苗膜脂过氧化及保护酶的影响[J].山东师大学报(自然科学版),1987(01):29-38.

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