| 69 | 0 | 33 |
| 下载次数 | 被引频次 | 阅读次数 |
盐胁迫影响植物生长发育的各个方面。谷子根系发达，具备高效活性氧清除机制，因此具有很强的抗旱性和抗盐性，然而，对于谷子耐盐机制仍然缺乏深入研究。研究表明，可变剪接在调控植物耐盐性方面发挥重要作用。本研究选取实验室前期大田实验筛选到的五个耐盐能力较强的谷子品种，分析其盐胁迫下表型、生物量及耐盐相关生理指标，筛选出耐盐能力较为突出的谷子品种869。基因表达分析证实，可变剪接调控基因SiCBP20在耐盐谷子品种869中的表达量与不耐盐品种相比，表达量更高，表明可变剪接在谷子耐盐能力调节方面发挥重要作用，这为解析可变剪接调控谷子耐盐性的分子机制提供了新的理论依据。
Abstract：Salt stress affects all aspects of plant growth and development. Foxtail millet, with its well-developed root system and efficient reactive oxygen species(ROS) scavenging mechanism, exhibits strong drought resistance and salt resistance. However, there is still a lack of in-depth study on the mechanism of its salt tolerance. Studies have shown that alternative splicing plays an important role in regulating salt tolerance in plants. In this study, five foxtail millet varieties with relatively strong salt tolerance were selected from previous field experiments in the laboratory, and their phenotypes, biomass and physiological indexes related to salt tolerance were analyzed under salt stress. Among them, variety 869 was identified as exhibiting particularly outstanding salt tolerance. Gene expression analysis confirmed that the expression level of the alternative splicing regulatory gene SiCBP20 is higher in salt-tolerant foxtail millet variety 869 than that in salt-intolerant foxtail millet varieties, indicating that alternative splicing plays an important role in regulating salt tolerance of foxtail millet. These findings provide a new theoretical basis for analyzing the molecular mechanism by which alternative splicing regulates salt tolerance of foxtail millet.
[ 1 ] Sachdev N,Goomer S,Singh L R.Foxtail millet:a potential crop to meet future demand scenario for alternative sustainable protein[J].Journal of the Science of Food and Agriculture,2021,101(3):831-842.
[ 2 ] Zhao J,Yu A,Du Y,et al.Foxtail millet (Setaria italica (L.) P.Beauv) CIPKs are responsive to ABA and abiotic stresses[J].Public Library of Science,2019,14(11):e0225091.
[ 3 ] Doust A N,Kellogg E A,Devos K M,et al.Foxtail millet:a sequence-driven grass model system[J].Plant Physiol,2009,149(1):137-141.
[ 4 ] Lata C,Gupta S,Prasad M.Foxtail millet:a model crop for genetic and genomic studies in bioenergy grasses[J].Critical Reviews in Biotechnology,2013,33(3):328-343.
[ 5 ] Peng R,Zhang B.Foxtail millet:a new model for C4 plants[J].Trends in Plant Science,2021,26(3):199-201.
[ 6 ] Aggarwal P R,Muthamilarasan M,Choudhary P.Millet as a promising C4 model crop for sustainable biofuel production[J].Journal of Biotechnology,2024,395:110-121.
[ 7 ] Patan S,Vallepu S,Shaik K B,et al.Drought resistance strategies in minor millets:a review[J].Planta,2024,260(1):29.
[ 8 ] Shrestha N,Hu H,Shrestha K,et al.Pearl millet response to drought:a review[J].Frontiers in Plant Science,2023,14:1059574.
[ 9 ] Zhang G,Liu X,Quan Z,et al.Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential[J].Nature Biotechnology,2012,30(6):549-554.
[10] Luo W,Tang Y,Li S,et al.The m6A reader SiYTH1 enhances drought tolerance by affecting the messenger RNA stability of genes related to stomatal closure and reactive oxygen species scavenging in Setaria italica[J].Journal of Integrative Plant Biology,2023,65(12):2569-2586.
[11] Qadir M,Quillérou E,Nangia V,et al.Economics of salt-induced land degradation and restoration[J].Natural Resources Forum,2014,38(4):282-295.
[12] Ran X,Wang X,Gao X,et al.Effects of salt stress on the photosynthetic physiology and mineral ion absorption and distribution in white willow (Salix alba L.)[J].Public Library of Science,2021,16(11):e0260086.
[13] Long J,Dong M,Wang C,et al.Effects of drought and salt stress on seed germination and seedling growth of Elymus nutans[J].PeerJ,2023,11:e15968.
[14] Pruthi R,Puram V R R,Ontoy J,et al.Genetics of yield component traits under salt stress at flowering stage and selection of salt tolerant prebreeding lines for rice improvement[J].Genetica,2022,150(5):273-288.
[15] Xu N,Lu B,Wang Y,et al.Effects of salt stress on seed germination and respiratory metabolism in different Flueggea suffruticosa genotypes[J].PeerJ,2023,11:e15668.
[16] Yu Z,Duan X,Luo L,et al.How plant hormones mediate salt stress responses[J].Trends in Plant Science,2020,25(11):1117-1130.
[17] Zhou H,Shi H,Yang Y,et al.Insights into plant salt stress signaling and tolerance[J].Journal of genetics and genomics,2024,51(1):16-34.
[18] Quintero F J,Ohta M,Shi H,et al.Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na+ homeostasis[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99(13):9061-9066.
[19] Yu J,Zhu C,Xuan W,et al.Genome-wide association studies identify OsWRKY53 as a key regulator of salt tolerance in rice[J].Nature Communications,2023,14(1):3550.
[20] Wang J,Nan N,Li N,et al.A DNA Methylation Reader-Chaperone Regulator-Transcription factor complex activates OsHKT1;5 expression during salinity stress[J].The Plant Cell,2020,32(11):3535-3558.
[21] Singh A,Roychoudhury A.Gene regulation at transcriptional and post-transcriptional levels to combat salt stress in plants[J].Physiologia Plantarum,2021,173(4):1556-1572.
[22] Filichkin S,Priest H D,Megraw M,et al.Alternative splicing in plants:directing traffic at the crossroads of adaptation and environmental stress[J].Current Opinion In Plant Biology,2015,24:125-135.
[23] Xu Z,Zhang N,Fu H,et al.Salt stress modulates the landscape of transcriptome and alternative splicing in date palm (Phoenix dactylifera L.)[J].Frontiers in Plant Science,2021,12:807739.
[24] Han M,Niu M,Gao T,et al.Responsive alternative splicing events of opisthopappus species against salt stress[J].International Journal of Molecular Sciences,2024,25(2).
[25] Terfa G N,Pan W,Hu L,et al.Mechanisms of salt and drought stress responses in Foxtail Millet[J].Plants,2025,14(8).
[26] Ribba T,Garrido-Vargas F,O′Brien J A.Auxin-mediated responses under salt stress:from developmental regulation to biotechnological applications[J].Journal of Experimental Botany,2020,71(13):3843-3853.
[27] He F,Niu M X,Feng C H,et al.PeSTZ1 confers salt stress tolerance by scavenging the accumulation of ROS through regulating the expression of PeZAT12 and PeAPX2 in Populus[J].Tree Physiology,2020,40(9):1292-1311.
[28] 熊良林，梁国鲁，郭启高，等.基因可变剪接调控植物响应非生物胁迫研究进展[J].植物学报，2025,60(3):435-448.
[29] Guo Y,Shang X,Ma L,et al.RNA-Binding Protein-Mediated alternative splicing regulates abiotic stress responses in plants[J].International Journal of Molecular Sciences,2024,25(19).
[30] Gao B,Chen M,Oliver M J.Alternative splicing:from abiotic stress tolerance to evolutionary genomics[J].International Journal of Molecular Sciences,2023,24(7).
[31] Feng J,Li J,Gao Z,et al.SKIP confers osmotic tolerance during salt stress by controlling alternative gene splicing in Arabidopsis[J].Molecular Plant,2015,8(7):1038-1052.
基本信息:
DOI：
中图分类号:S515
引用信息:
[1]张燕玲,赖羚珺,成茗,等.可变剪接在谷子耐盐性中的作用[J].山东师范大学学报(自然科学版),2025,40(03):263-270.
基金信息:
国家自然科学基金资助项目(32272040)