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脂质转移蛋白(Lipid transfer protein, LTP)是一类在植物细胞中普遍存在的小分子转运蛋白，主要负责脂类的转运和各种生化反应产物的输送。本研究在前期鉴定到的谷子干旱胁迫响应基因SiLTP1、并获得该基因的谷子阳性转基因株系的基础上，对盐胁迫下其脂质组学进行分析。通过GC-MS检测，显示双半乳糖基二酰甘油(DGDG)和单半乳糖基二酰甘油(MGDG)在谷子品种Ci846和过表达SiLTP1株系幼苗中的含量最高。在200 mmol·L-1的NaCl处理后，过表达SiLTP1的谷子幼苗叶片中DGDG/MGDG的含量较Ci846的高。同时，磷脂酰甘油(PG)和甘油二脂(DG)等脂质分子的含量也有所增加。基于以上结果，显示脂质转移蛋白可通过调控磷脂和脂类分子等在SiLTP1过表达谷子幼苗中的含量，进而参与谷子盐胁迫响应。本研究为系统解析脂质转移蛋白参与谷子盐胁迫响应的机制、进而培育谷子耐盐品种提供了理论依据及候选基因资源。
Abstract：Lipid transfer proteins(LTPs) are a class of small molecular transport proteins widely present in plant cells, primarily responsible for the transport of lipids and the delivery of various biochemical reaction products. In this study, based on the previously identified drought stress-responsive gene SiLTP1 in millet and the obtained positive transgenic millet lines overexpressing SiLTP1, lipidomic analysis under salt stress was performed. GC-MS analysis revealed that the levels of digalactosyldiacylglycerol(DGDG) and monogalactosyldiacylglycerol(MGDG) were the highest in the seedlings of millet variety Ci846 and the SiLTP1-overexpressing lines. After treatment with 200 mmol·L-1 NaCl, the levels of DGDG/MGDG in the leaves of SiLTP1-overexpressing millet seedlings were higher than those in Ci846. At the same time, the levels of phosphatidylglycerol(PG) and diglycerides(DG) also increased. Based on these results, it is suggested that lipid transfer proteins can modulate the content of phospholipids and lipid molecules in the SiLTP1-overexpressing millet seedlings, thereby participating in the response to salt stress in millet. This study provides theoretical support and candidate gene resources for the systematic analysis of the mechanisms through which lipid transfer proteins participate in millet′s salt stress response, offering a foundation for the development of salt-tolerant millet varieties.
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基本信息:
DOI：
中图分类号:S515
引用信息:
[1]孟凡花,沈傲,刘敏等.盐胁迫下脂质转移蛋白SiLTP1转基因谷子的脂质组学分析[J].山东师范大学学报(自然科学版),2025,40(02):162-170.
基金信息:
国家自然科学基金资助项目(32171955,32201736); 山东省农业良种工程资助项目(2021LZGC006); 山东省重点研发计划资助项目(2021LZGC025); 山东省农业科学院农业科技创新工程资助项目(CXGC2023F13)